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. 2007 Jul 1;21(13):1598–1602. doi: 10.1101/gad.1561307

Figure 4.

Figure 4.

BRI1 signaling is induced by BFA treatment. (A) A highly sensitive Arabidopsis green cell suspension line shows dephosphorylation of BES1 (green, above) and suppression of early-response gene, DWF4 (green, below) already at 1 nM BL for 30 min. (Middle) BFA treatment alone leads to an efficient activation of BES1 (red) and suppression early-response genes. External BL enhances the BFA effect on BES1 (yellow, above) and DWF4 (yellow, below). (B) Enhancement of BR signaling by BFA can also be observed in BRI1-overexpressing seedlings, when treating with low amounts of BL (10 nM) for 4 h, which leads to only half-maximal dephosporylation of BES1 (above) and a twofold reduction in DWF4 levels (green, below). Adding BFA (100 μM) after 30 min of BL pretreatment significantly enhances BES1 activation (above) and DWF4 suppression (red, below).