Figure 5.

Induced BRI-YFP expression in cultured roots. (A) BFA effects can also be observed in intact organs using a bri1-null mutant root culture containing heat-shock-inducible BRI1-YFP. In this system, the effects of BFA on localization and signaling can be simultaneously assessed. Top panel shows BRI-YFP signals, 8 h after a 30-min heat-shock or control treatment. BFA treatment (100 μM) started 4 h after heat shock. Middle panel shows BRI1-YFP levels as detected on immunoblot with α-BRI1 after same treatments as above. Bottom panel shows heat-shock-induced suppression of DWF4 (gray) and its further reduction by BFA treatment (red). Bar, 10 μm.