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. 2007 Jul 1;21(13):1615–1620. doi: 10.1101/gad.1534807

Figure 4.

Figure 4.

Ina-1 expression is necessary for normal and perpetual DTC migration. Images of strain NG2517, an INA-1∷GFP translational fusion, show diminishing GFP fluorescence between L4 (A) and adult DTCs (B). (C) INA-1∷GFP is maintained throughout egg-laying in the adult vab-3 mutants. (D,E) Gonads were dissected from age-matched NG2517 wild-type or vab-3 mutants expressing INA-1∷GFP. Fluorescence images were captured at identical settings. Arrows point to DTCs (A–E), and arrowheads point to vulva (A,B). RNAi against ina-1 on N2 (F) or vab-3(e1796) (G) nematodes stops DTC migration. Simultaneous RNAi against ced-10 and mig-2 (H) on a vab-3 mutant prevents perpetual migration. Dashed lines trace gonad arms, and arrowheads mark the DTCs. Bars: all panels except D,E, 25 μm; D,E, 12.5 μm.