Figure 1.

A functional genomics strategy revealed cwo as a clock component. (A) Genome-wide tissue-specific knockdown analysis of clock-controlled genes in Drosophila. UAS-IR transgenic lines to express dsRNA for the target gene under the control of UAS were established (Pili-Floury et al. 2004). Usually two independent insertion lines are established for one target gene. Each of the UAS-IR lines was mated to tim(UAS)-gal4 to drive the expression of dsRNA specifically within clock cells. The locomotor activity of RNAi transgenic flies for 133 candidates among 200 clock-controlled genes was recorded in DD. UAS-TATA sequence or UAS sequence (yellow rectangle), SV40 late polyA site (white rectangle), ∼500-bp fragment of a target gene (red arrow), a tim promoter region (green rectangle), and gal4 gene (pink arrow) are represented. (B) Typical locomotor activity in wild-type, knockdown flies of known clock genes (left) and five new candidates (right). The names of the knocked-down genes are described at each actogram.