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Delineation of the minimal DLC2-SAM domain. (a) By sequence homology, the DLC2-SAM domain is defined by exons 2–4. Of four protein fragments expressed, three were suitable for further biophysical analyses. (b) Far UV CD spectra. (c) Thermal stability was determined by monitoring ellipticity at 220 nm, a wavelength characteristic of α-helical secondary structure.
