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Biochemical characterization of anti-LDH-5 (A) and anti-PDH (B) monoclonal antibodies by SDS-PAGE. 1A) 1, rabbit heart; 2, rabbit muscle; 3, human LDH-1 and 4, human hippocampal extracts. 1B) 1–3, human hippocampal extracts (2, mitochondrial fraction, 3, cytosolic fraction), 4–6, human occipital cortex extracts(5, mitochondrial fraction, 6, cytosolic fraction), 7, PDH from porcine heart and 8, LDH-5 from rabbit muscle (absence of positive band at 35 kDa omitted).
