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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Dev Biol. 2007 Feb 21;305(1):300–311. doi: 10.1016/j.ydbio.2007.02.019

Figure 4.

Figure 4

Effect of oocyte co-culture on development of competence to undergo expansion (A) and expression of expansion transcripts (B) in oocytectomized PAGCs cultured in control medium or co-cultured with FGO (1 oocytes/μl) for 6 days (culture period). At the end of the culture period, PAGCs were removed from the agarose and treated with control medium or EGF (10 ng/ml) for 6 hours (mRNA levels) or 15 hours (expansion assay). Freshly isolated oocytes were added to all PAGC treatment groups during the test period only (2 oocytes/μl). Test period treatments, including the oocytes added to provide CEEFs, are shown in the both 4A and 4B. *Denotes value different from control as determined by Student's t-test, P<0.05.