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Determination of the transcriptional start sites of the puf operon (A) and hmuO (B). Primer extension analyses were performed with RNAs extracted from ORS278 strain cultured under a dark–light illumination cycle (P) or in darkness (D). As a reference, a DNA sequencing ladder is shown (lanes G, C, T, and A). The sequences were obtained with the same primers used for the primer extensions. The transcription start points (+1) are indicated by arrows.
