FIG. 2.

GFP-bud6-1p stains the SPB in the absence of dynein or microtubules. (A) Homozygous dynein deletion strain PY23643x3297 (Table 1) expressing GFP-bud6-1p from pDA257. (B) Wild-type diploid strain FY23x86 carrying pDA257 was treated with nocodazole, and GFP-bud6-1p fluorescence was observed at various times; the image shown is of cells after 105 min of exposure to nocodazole, at which point 94% of cells had medium to large buds.