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GFP-bud6-1p is present as SPB-like dots in nud1-44 cells. Both (A) wild-type NUD1 (IAY522) and (B) mutant nud1-44 (IAY520) (Table 1) cells containing pDA257 maintain GFP-bud6-1p at the SPB after 4 h at the nud1-44 restrictive temperature of 37°C.
