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The SPB outer plaque is required for localization of Bud6p to the SPB. (A and B) Strain AHY5 carrying pDA257 was treated with α-factor for 3 h as described in Materials and Methods, and cells were observed for GFP-bud6-1p (A) and Spc42p-ECFP (B). (C and D) The cnm67 mutant strain AHY13 carrying pDA257 was observed for GFP-bud6-1p (C) and Spc42p-ECFP (D). Note that Spc42p-ECFP is still able to stain the SPB, as the central plaque is not affected in cnm67 null cells.
