FIG. 6.

The role of Bud6p in pulling the spindle through the neck. (A) A bud6Δ mutation does not exacerbate the growth defects of kar9 or arp1 (dynactin) mutants, indicating that Bud6p does not have an important role in either Kar9p or dynein-mediated spindle position; doubling times of the indicated strains are expressed in hours. (B) A bud6 mutation does not increase the frequency with which cells misposition their spindles in wild-type, arp1, or kar9 mutant cells (expressed as percent anaphase spindles in the mother). The strains used for these experiments were YJC3677, -3681, -3685, -3689, -3693, -3697, -3701, and -3705 (Table 1).