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bud6Δ cells are not defective for the spindle position checkpoint. Cdc14-GFP localization was followed in wild type (BHY444), bud6Δ (BHY443), and bub2Δ (BHY482) haploid cells before (0 h) or 2 h after addition of nocodazole. Nucleolar Cdc14-GFP was scored as a single cluster in mother or daughter cells (hatched) versus two or more clusters in mother and daughter cells (white); “diffuse” cells (black) had no obvious nucleolar staining. n > 150 cells for each time point.
