TABLE 2.
Plasmids used in this study
| Plasmid | Markers | Source or reference |
|---|---|---|
| pDP128 | Act1p-GFP-BUD61-788-Act1t URA3 | D. Pruyne |
| pXH133 | URA3 SPC42-ECFP | P. Sorger |
| pAH1 | LEU2 SPC42-ECFP | This study |
| pTD125 | Act1p-GFP-MCS-Act1t URA3 Cen | T. Doyle |
| pDAb204 (pRB2190) | Act1p-GFP-BUD61-788-Act1t URA3 Cen | 5 |
| pDA257 | Act1p-GFP-bud6-11-479-Act1t URA3 Cen | 31 |
| pBH432 | Act1p-GFP-Bfa1-Act1t URA3 Cen | This study |
| pBH442 | Act1p-GFP-Bub2-Act1t URA3 Cen | This study |
| pBH444 | Act1p-GFP-Tem1-Act1t URA3 Cen | This study |
| pBH448 | Act1p-GFP-Lte1-Act1t URA3 Cen | This study |
| pFA6a-kanMX6 | Kanr cassette P TEF T TEF | M. Longtine |
| pBJ1351 | TUB1-GFP::LEU2 | 57 |
| pBJ1517 | GAL1-BUD6 | 64 |