Figure 1.
Dose-dependent effect of 2ME (A) and BE-3-3-3 (B) on cell proliferation and the combination index (CI) plot (C). Experiments were conducted in the presence of 10 nM E2. Cell number was determined on day 4 of treatment by trypan blue exclusion assay. For combination experiments, six doses of 2-ME and BE-3-3-3 were used from serial dilutions covering the IC50 values. CI analysis was conducted as described in Materials and Methods. CI were < 1 for all combinations. Data are ± SEM from three separate experiments.