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. Author manuscript; available in PMC: 2008 Jun 8.
Published in final edited form as: Cancer Lett. 2006 Dec 20;250(2):311–322. doi: 10.1016/j.canlet.2006.10.027

Figure 4.

Figure 4

Effect of E2, 2ME and BE-3-3-3 on Akt phosphorylation levels in MCF-7 cells. Cells were treated with E2, or E2 + 2ME/BE-3-3-3 combinations for 24 h. Cells were harvested and the lysate was analyzed by sequential Western blots using antibodies specific to phospho-Akt, total Akt, PTEN and β-actin. Similar results were obtained from 3 separate experiments.