TABLE 1.
Half-times of dissociation for various treatments and glucocorticoid receptor mutants
| Receptor | Treatment | GR localization | t1/2 (s)a |
|---|---|---|---|
| GFP-GR | Nucleoplasm | 0.425 ± 0.040 (n = 15) | |
| GFP-GR | DEX | Nucleoplasm | 1.357 ± 0.153 (n = 11) |
| GFP-GR | DEX | Array | 1.597 ± 0.088 (n = 15) |
| GFP-GR | DEX-mes | Nucleoplasm | 0.917 ± 0.089 (n = 17) |
| GFP-GR | DEX-mes | Array | 1.404 ± 0.100 (n = 19) |
| GFP-GR | RU486 | Nucleoplasm | 0.798 ± 0.069 (n = 11) |
| GFP-GR | RU486 | Array | 0.819 ± 0.064 (n = 17) |
| GFP-GR | DEX-Cl | Nucleoplasm | 0.985 ± 0.089 (n = 15) |
| GFP-GR | Corticosterone | Nucleoplasm | 0.829 ± 0.119 (n = 6) |
| GFP-GR | Corticosterone + MG-132 | Nucleoplasm | 1.207 ± 0.144 (n = 7) |
| GFP-N525 | Nucleoplasm | 0.560 ± 0.035 (n = 12) | |
| GFP-N525 | Array | 0.691 ± 0.058 (n = 24) | |
| GFP-407C | Nucleoplasm | 0.453 ± 0.038 (n = 10) | |
| GFP-407C | DEX | Nucleoplasm | 0.956 ± 0.067 (n = 12) |
| GFP-407C | DEX | 1.340 ± 0.095 (n = 18) |
a
Values are means ± SEM. n, number of cells assayed.