FIG. 2.

Nucleoplasmic NF7 exists as a homotrimer. (A) Flow chart indicating the fractionation of nucleoplasmic NF7, which was followed through each step of purification by Western blot analysis with MAb 37-1A9. (B) Sypro orange-stained gel with each lane containing an equal volume of elution fractions 5 to 13 from the MonoS column. The major band with an apparent molecular mass of 80 kDa of fraction 09 was identified as NF7 by tandem mass spectrometry. The values on the left are molecular sizes in kilodaltons. (C) Fraction 09 from the MonoS column was further fractionated on a Sephacryl 200 column, and NF7 eluted at 240 kDa.