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. 2007 Jan 29;27(7):2615–2624. doi: 10.1128/MCB.01968-06

FIG. 6.

FIG. 6.

NF7 trimerization occurs in the cytoplasm. Western blot assay showing the cellular distribution of two modified forms of NF7 upon expression in stage V oocytes. Both proteins were tagged with the HA epitope, and MAb 3F10 was used for their detection. Whether the transcripts coding for ΔNLS (80 kDa) and ΔC448 (60 kDa) were injected individually or coinjected is indicated by the plus and minus signs above the lanes. Cytoplasmic and nuclear protein samples were prepared 18 h after injection, and each lane corresponds to either five cytoplasms or 10 nuclei. The values on the left are molecular sizes in kilodaltons.