FIG. 2.

L-Pik3r1KO hepatocytes are JNK resistant. Primary hepatocytes were isolated from lox/lox mice or L-Pik3r1KO mice, infected with a JNK1-expressing adenovirus or a control LacZ adenovirus at an MOI of 100, and then treated with saline or 100 nM insulin after 12 h of serum starvation. Western blot assays were then performed against lysates from the treated hepatocytes with the indicated antibodies. The phosphospecific antibody blots were stripped and reprobed with the appropriate antibodies to determine the total levels of the corresponding proteins.