FIG. 7.

Role of TGN and vesicular transport in furin and nSMase activation by TNF-α. Brefeldin A (10 μM) or monensin (10 μM) was incubated with SMC for 6 h before stimulation with TNF-α and furin (A) or nSMase (B) activity determination. Brefeldin and monensin controls were done simultaneously and exhibited no toxicity for the cells during the time course of the experiment (not shown). Results are means ± SEM from three separate experiments. *, P < 0.05 (comparison between treated and untreated cells at time zero).