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HA-Rkr1 localizes to the nucleus. Indirect immunofluorescence experiments were performed with transformants of a wild-type strain (FY632) expressing HA-Rkr1 (A and B) or untagged Rkr1 (C and D). Localization of the triple HA epitope was detected with Cy3-coupled secondary antibody (A and C), and nuclear and mitochondrial DNAs were detected with DAPI stain (B and D). HA-Rkr1 and untagged Rkr1 were expressed from the CEN/ARS plasmids pMB11 and pPC65, respectively.
