TABLE 3.
Relevant genotypea | Phenotypeb |
---|---|
rtf1Δ3 rkr1Δ | SD− |
rtf1Δ4 rkr1Δ | SD− |
rad6Δ rkr1Δ | Slow growth, SD−, Gly− |
bre1Δ rkr1Δ | SD−, Gly− |
lge1Δ rkr1Δ | SD− |
set1Δ rkr1Δ | SD−, HUS, NaCl− |
set1Δ rkr1-C1508A | SD−c |
dot1Δ rkr1Δ | None |
set2Δ rkr1Δ | None |
spt10Δ rkr1Δ | Dead |
spt21Δ rkr1Δ | Slow growth |
Double mutants, in the order listed, were generated from the following genetic crosses (unless stated otherwise): KY1166 and KY1174, KY1166 and KY1175, KY1167 and FY623, KY1171 and KY968, none (lge1Δ was created in an RKR1/rkr1Δ diploid [KY1173] prior to sporulation and tetrad dissection), KY1168 and KY907, none (the set1Δ rkr1-C1508A double mutant [KY1222] was generated by transformation), KY1168 and KY903, KY1168 and KY912, FY896 and KY1168, and FY2199 and KY1168.
All phenotypes listed correspond to the synthetic phenotypes observed for the double mutant strains. Definitions of phenotypes are as follows: SD−, poor growth on SD medium; slow growth, small colonies after 7 days of growth at 30°C on YPD; Gly−, inviable on YPG medium; HUS, sensitive to 100 mM hydroxyurea; NaCl−, sensitive to 1.4 M NaCl.
Growth on SD medium was the only phenotype tested for this double mutant.