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. 2007 Feb 26;27(9):3470–3480. doi: 10.1128/MCB.00659-06

FIG. 1.

FIG. 1.

Conditional targeting of the Gabpα locus. (A) Schematic of wild-type, targeted, floxed, and conditional-knockout Gabpα alleles. A floxed Neomycin (neo) cassette was inserted within intron 2, and a single loxP site immediately upstream of exon 2. The positions of the Southern blot probe, PCR primers and restriction sites for BamHI (B), BalI (Bl), EcoRI (E), and HindIII (H) are shown. (B) Southern blot analysis of genomic DNA from G418-resistant ES cells (clone numbers are shown), using a BamHI digest and an external intron 3 probe to detect 7-kb wild-type and 5-kb targeted fragments. (C) PCR screening of puromycin-resistant targeted ES cell clones following transient Cre transfection was performed with primers G1f and G1r, spanning Gabpα introns 1 and 2 flanking the site of insertion of the neo cassette, generating 1.8-kb wild-type (wt), 1.9-kb floxed (loxP), and 1.5-kb knockout (ko) allele products.