FIG. 2.

Specific loss of Gabpα expression in skeletal muscle. (A) PCR screening of genomic DNA extracted from organs of wild-type (+/+), heterozygous (+/−), and homozygous knockout (−/−) mice. A common 5′ primer within intron 1 (G2f) and 3′ primers within exon 2 (G2r) and intron 2 (G1r) generated products of 620 bp, 660 bp, and 740 bp, representing wild-type, floxed, and knockout alleles, respectively. The knockout is represented by an asterisk in each case where no wild-type allele was detectable. (B) Western blot analysis of Gabpα (58-kDa) and β-tubulin (50-kDa) protein levels in skeletal muscle tissues and primary skeletal muscle cell cultures from Gabpα skeletal-muscle-specific knockout mice (−/−) relative to homozygous floxed (+/+) littermates. Severely reduced Gabpα protein levels were observed in whole muscle tissue lysates of Gabpα mutant mice and complete loss of Gabpα protein expression in Gabpα mutant primary skeletal muscle cells derived from mice at postnatal days 2 and 3.