Skip to main content
. 2007 Feb 26;27(9):3481–3488. doi: 10.1128/MCB.02088-06

FIG. 4.

FIG. 4.

Loss of sister cohesion in Cdc20 and securin double mutants. (A) Micrograph of a litter from an intercross between securin−/− Cdc20+/gt mice at E3.5. Asterisks indicate embryos arrested at the two-cell stage. (B) In vitro culture of two-cell embryos. E1.5 embryos were isolated, cultured, and genotyped. The double homozygous mutants are marked by asterisks. Immunofluorescence staining of cyclin B1. E1.5 embryos were stained after 18 h of in vitro culturing. Two-cell-stage embryos were cultured for 12 h. Arrows indicate polar bodies. (E) Chromosome spread of a two-cell embryo after 18 h of in vitro culturing. The embryo was obtained from an intercross between securin−/− Cdc20+/gt mice. Scale bars in panels A and B, 20 μm; in panels C to E, 5 μm.