FIG. 8.
Decreased expression of cell cycle regulators CDKN2a and CDKN1b in Ets1ΔVII mice is associated with increased proliferation. (A) RT-PCR analysis of thymocyte and splenocyte mRNA show diminished expression of CDKN1b transcript levels in Ets1ΔVII mice, hypoxanthine phosphoribosyltransferase (HPRT) loading control (30 cycles). (B) Western blot analysis of total protein from isolated thymocytes and splenocytes shows diminished expression of p27Kip1 (CDKN1b) protein in Ets1ΔVII thymocytes. β-Actin loading control. (C) RT-PCR analysis of thymocyte and splenocyte mRNA show diminished expression of CDKN2a transcript levels in Ets1ΔVII mice. HPRT loading control (30 cycles). Ets1 genotypes are given above each blot.