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. 2007 Mar 5;27(9):3353–3366. doi: 10.1128/MCB.01871-06

TABLE 1.

Relative quantification of Ets1 transcripts in wild-type and targeted micea

Thymocyte subpopulation Result for indicated genotype
ΔΔCT Relative expression
Ets1+/+
Ets1ΔVII/ΔVII
Expt 1 Expt 2 Mean ΔCT Expt 1 Expt 2 Mean ΔCT
ETS1full
    DN 36.71 37.14 36.93 11.85 40.00 40.00 40.00
    DP 36.16 36.23 36.20 9.79 40.00 40.00 40.00
    CD8 37.18 38.19 37.69 11.91 40.00 40.00 40.00
    CD4 36.45 37.41 36.93 11.55 40.00 40.00 40.00
ETS1ΔVII
    DN 31.69 31.85 31.77 6.70 36.16 34.63 35.40 5.96 −0.74 1.670
    DP 31.36 31.30 31.33 4.93 31.18 32.19 31.69 5.05 0.12 0.920
    CD8 32.36 32.61 32.49 6.71 31.06 30.82 30.94 5.70 −1.02 2.028
    CD4 31.67 32.02 31.85 6.07 31.71 31.28 31.50 6.25 0.18 0.883
a

DN, double negative; DP, double positive; CD8, single-positive CD8; CD4, single-positive CD4 thymocytes.