FIG. 1.

Targeted disruption of the Pcyt2 gene. (A) The targeting vector was created by replacing a 2.8-kb fragment, including exons 1 to 3 and the translational start site, with a neomycin (Neo) cassette. Short and long homologous arms of 1.4 and 8 kb, respectively, flank the neomycin cassette. Genotyping primer locations are indicated. (B) PCR confirmation of two recombinant ES clones, 286 and 883, using primers AT1 and N1 (1.5 kb). (C) Genotyping of mouse genomic DNA using a common forward primer, FP, and two specific reverse primers, RP and N1, for the wild-type and knockout alleles, respectively.