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. 2007 Feb 26;27(10):3569–3577. doi: 10.1128/MCB.01447-06

FIG. 2.

FIG. 2.

Time course of IRS-1 and IRS-2 activation by insulin, IGF-II, and IGF-I. Cells were treated as described in the legend to Fig. 1, and the lysates were immunoprecipitated (IP) with anti-IRS-1 and IRS-2 antibodies, followed by Western immunoblotting with anti-IRS and PY20 antibodies. (A) IRS-1 activation in R− IR-A cells; (B) IRS-1 activation in R− IR-B cells; (C) IRS-2 activation in R− IR-A cells; (D) IRS-2 activation in R− IR-B cells. Representative blots are shown beneath each graph. In each case, the PY20 signal was normalized for IRS-1 or IRS-2 levels as determined by blotting with the respective antibody separately for each sample. Error bars represent SEM of three independent experiments. The bottom portion of the gel image in each case corresponds to a single representative IRS-1 or IRS-2 control blot. max, maximum; pIRS-1, phosphorylated IRS-1; pIRS-2, phosphorylated IRS-2.