FIG. 6.

Effect of IR transfection on IGF-I and Long R3 IGF-I activation of IRS-2. Control R⁻ cells and RI⁻ R-A and R⁻ IRB cells were treated with 10 nM IGF-I (open bars) or Long R3 IGF-I (closed bars) and analyzed as described in the legend to Fig. 2. Error bars represent SEM of three independent experiments. Statistical significance shown is in relation to levels of phosphorylation in IGF-I-treated R⁻ cells or, where indicated, between the bracketed samples. P values are represented as follows: **, P < 0.01; ***, P < 0.001. NS, not significant; max, maximum; IP, immunoprecipitation; WB, Western blotting.