FIG. 2.

Developmental phenotypes associated with mutations in Dmp52. (A) Wt and mrn¹/mrn¹ mutant third-instar larvae. Note the reduced side of the mutant organism compared to the wt. (B) Wt and EP3605/EP3605 mutant pupae. Note the reduced size of the mutant pupa. (C) Some EP3605/EP3605 and EP3605/mrn¹, mrn³, and mrn⁵ flies can go through metamorphosis but die at different stages before completing development. (D and E) Wt and EP3605/EP3605 pharates, respectively. The mutant organisms have cuticular defects, as well as fragile and deformed bristles (brittle-bristle phenotype). The defects are indicted by arrows. (F and G) mrn¹/mrn¹ and heteroallelic EP3605/mrn³ larva and pupa, respectively, with melanotic tumors indicated by arrows. (H and I) Presence of melanization in the imaginal ring midgut of heteroallelic EP3605/mrn³ larvae (arrows). This phenotype was always present in the imaginal ring region of the larval gut and extended from this point in both directions. (J) Wt and EP3605/mrn³ adult females. Each genotype is indicated. Note the difference in size between the organisms. (K) Ovarioles from a heterozygous EP3605/+ female. Note that the egg chambers (E. Ch.), nurse cells (N.C.), and oogenesis stages are normal. (L) Ovarioles from an EP3605/EP3605 female. Note that the first oogenesis stages are normal, but the eggs are deformed, apparently resulting from an aberrant chorion (egg shell). (M and N) Ovarioles from heteroalelic EP3605/mrn¹ and EP3605/mrn³ females, respectively. Note the aberrant egg chambers, the disrupted nurse cells, and the presence of picnotic nuclei in the eggs (indicated by arrows). (O) Testis from a heterozygous mrn¹/+ male. Note that all spermatogenesis stages are present. (P) Testes from an EP3605/mrn¹ organism. Note that the testes are aberrant and lack cells progressing through spermatogenesis. The tissue preparation nuclei were visualized by DAPI staining.