FIG. 3.

XPB and XPD protein levels are reduced in the Dmp52 mutants. Wt and heteroallelic mutant third-instar larval salivary glands were costained with either XPB/histone H3, XPB/TBP, or XPD/histone H3 and XPD/PBT antibodies. (A) Costaining and quantification of the XPB/histone ratio. Note that the XPB levels are reduced at least twofold in the mutant nuclei. (B) Costaining and quantification of the XPB/TBP ratio. XPB levels are reduced compared with the control. (C) Costaining and quantification of the XPD/histone ratio. (D) Costaining and quantification of the XPD/TBP ratio. In EP3605/mrn¹ and EP3605/mrn³ heteroallelic flies, the XPD levels are reduced. For quantification of fluorescence, we followed the protocol reported previously (31) (see Materials and Methods). At least 10 nuclei for each condition were analyzed. The error bars indicate standard errors. For details, see the text.