TABLE 2.
Rescue of the sterility phenotype in heteroallelic organisms by different transgenic flies
| Mutant genotypea | Sterility rescue by transgenic fly (%)b
|
||||
|---|---|---|---|---|---|
| Dmp52-1 | Dmp52-2 | hp52-3 | hp52-4 | Dmp52-mutc | |
| EP3605/EP3605 | 85 | 76 | 95 | 59 | 0 |
| mrn5/EP3605 | 75 | 86 | 100 | 77 | 0 |
| mrn1/EP3605 | 100 | 80 | 0 | 0 | 0 |
| mrn3/EP3605 | 75 | NA | 75 | 87 | NA |
a
Homozygous EP3605/EP3605 and heteroallelic (EP3605/mrn1, mrn3, and mrn5) adults are 100% sterile.
b
The percentage represents the individual flies that were fertile and able to generate progeny that developed to larval stage. The total numbers of flies tested were between 10 and 100 for each condition. The numbers of males and females are merged to give a general percentage. There was not bias in sex ratios (data not shown). NA, not analyzed.
c
The Dmp52-mut line is a transgenic line expressing the double point mutant (E340K-R344E).