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. 2007 Mar 12;27(10):3743–3749. doi: 10.1128/MCB.01561-06

FIG. 5.

FIG. 5.

Transcriptional activity of BSX1A in yeasts. (A) Schematic representation of full-length or truncated (ΔC or ΔN) BSX1A fused to GAL4DBD. HD, homeodomain. (B) (Top) β-Gal activity measured by a liquid assay using o-nitrophenol-β-d-galactoside (ONPG) as a substrate. The values of the ONPG assay are indicated in Miller units. (Bottom) Results of a β-Gal filter assay using X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) as a substrate. Vec, vector. (C) Western analysis using anti-GAL4DBD antibody to confirm GAL4DBD fusion protein expression.