FIG. 8.

Pes1 is required for nucleolar localization of Bop1. Bop1-HA expressed in (A) H1299 cells and (B) TGR-1 cells was detected by indirect immunofluorescence assay. Coexpression of luciferase (control), WDR12, and Pes1 is indicated. Recombinant proteins were induced by doxycycline for 30 h, and cells were fixed with methanol-acetone. Bop1 was stained by guinea pig polyclonal antibodies specific for mouse Bop1 (poly-α-Bop1). Nuclei were counterstained with 4′,6′-diamidino-2-phenylindole (DAPI).