TABLE 2.
trans phosphorylation of ABCDE on one of two synapsed DNA-PK complexes substantially relieves the end-processing defect associated with blocking ABCDE phosphorylationa
| Transfectant | No. of sequences | Avg nucleotide loss/joint | % Complete ends | Joints with SSH | % Complete ends with P segments | % P segments > 2 bp |
|---|---|---|---|---|---|---|
| Wild type | 50 | 6.5 | 22 (22/100) | 64 (32/50) | 55 (12/22) | 8 (1/12) |
| RAGs only | 42 | 22.2 | 37 (31/84) | 38 (16/42) | 87 (27/31) | 71 (20/28) |
| K>R | 45 | 9.5 | 28 (25/90) | 56 (25/45) | 56 (14/25) | 21 (3/14) |
| ABCDE | 50 | 1.9 | 58 (58/100) | 18 (9/50) | 36 (18/50) | 22 (4/18) |
| ABCDE+K>R | 59 | 5.7 | 26 (31/118) | 58 (34/59) | 16 (5/31) | 0 (0/5) |
| K>R+PQR | 41 | 10 | 12 (10/82) | 68 (28/41) | 20 (2/10) | 0 (0/2) |
| PQR | 45 | 10.2 | 13 (12/90) | 73 (33/45) | 25 (3/12) | 0 (0/3) |
a
Data were calculated from sequences of individual coding joints. SSH, short sequence homologies.