TABLE 2.
The sy262 mutation predominantly acts through Ras rather than Rac
| Genotypea | Vulval inductionb | % Muvc | % Vuld | ne | P valuef |
|---|---|---|---|---|---|
| Wild type | 3.00 | 0 | 0 | 24 | |
| sy262 | 3.00 | 0 | 0 | 20 | |
| let-23(rf) | 0.23 | 0 | 100 | 20 | |
| let-23(rf); sy262 | 3.80 | 68 | 0 | 22 | |
| let-60(rf)/+ | 3.00 | 0 | 0 | 20 | |
| let-23(rf); let-60(rf)/+; sy262 | 1.25 | 0 | 80 | 22 | <0.00001 to let-23(rf); sy262 |
| rac-2(null) | 3.00 | 0 | 0 | 20 | |
| let-23(rf); rac-2(null); sy262 | 3.65 | 60 | 0 | 20 | 0.50 to let-23(rf); sy262 |
| ced-10(rf) | 3.00 | 0 | 0 | 20 | |
| let-23(rf); ced-10(rf); sy262 | 3.90 | 70 | 5 | 20 | 0.68 to let-23(rf); sy262 |
| mig-2(null) | 3.00 | 0 | 0 | 20 | |
| let-23(rf); sy262; mig-2(null) | 2.85 | 25 | 25 | 20 | 0.002 to let-23(rf); sy262 |
| let-23(rf); rac-2(RNAi); sy262; mig-2(null) | 2.83 | 30 | 30 | 18 | 0.97 to let-23(rf); sy262; mig-2(null) |
| let-23(rf); ced-10(RNAi); sy262; mig-2(null) | 2.55 | 21 | 47 | 19 | 0.45 to let-23(rf); sy262; mig-2(null) |
| let-23(rf); ced10(RNAi); rac-2(RNAi); sy262; mig-2(null) | 2.68 | 30 | 40 | 20 | 0.64 to let-23(rf); sy262; mig-2(null) |
let-23(rf) = let-23(sy1), let-60(rf)/+ = let-60(n1876) unc-22(s7)/dpy-20(e1282), rac-2(null) = rac-2(ok326), ced-10(rf) = ced-10(n1993), and mig-2(null) = mig-2(mu28). In the mig-2(null) strains, sy262 was linked to unc-46(e177). In all other strains, sy262 was linked to him-5(e1490). By themselves, the unc-46 and him-5 mutations do not affect vulval development in let-23(rf) animals.
Average number of vulval progenitor cells adopting vulval fates. Wild type is three. The maximum is six.
The percentage of animals that have more than three vulval progenitor cells adopting vulval fates.
The percentage of animals that have fewer than three vulval progenitor cells adopting vulval fates.
n, number of animals examined.
Statistical significance of the vulval induction value as determined by a two-tailed Student's t test.