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. 2007 Mar 19;27(10):3578–3588. doi: 10.1128/MCB.01808-06

TABLE 1.

Oligonucleotides used in this studya

Name Sequence of oligonucleotide Orientation Positionb
MMTR 1F (EcoRI) 5′-GAATTCTA ATGGCTACGGGC-3′ Forward +58
MMTR 1R (XhoI) 5′-CTCGAGAGCATCAAACACTGG-3′ Reverse +718
MMTR 2F (EcoRI) 5′-GAATTCTA GGGCATGAGAGACGG-3′ Forward +719
MMTR 2R (XhoI) 5′-CTCGAGTGG TTTCTTGGCTTTC-3′ Reverse +1462
MMTR 1F (NotI) 5′-GCGGCCGCATGGCTACGGGC-3′ Forward +58
MMTR 2F (NotI) 5′-GCGGCCGCGGGCATGAGAGACGG-3′ Forward +719
MAT1 primer1 (EcoRI) 5′-CTGAATTCATGGACGATCAGGG-3′ Forward +102
MAT1 primer2 (XbaI) 5′-TCTAGATTAACTGGGCTGCC-3′ Reverse +1029
MAT1 primer3 (XbaI) 5′-TCTAGAATCCACATTGTTGG-3′ Reverse +444
MAT1 primer4 (EcoRI) 5′-CTGAATTCACCAACAATGTGGA-3′ Forward +429
MAT1 primer5 (XhoI) 5′-CTCGAGTAGAATCTGCTGCAG-3′ Reverse +627
MAT1 primer6 (EcoRI) 5′-CTGAATTCAAGCAGGCTTTTTTA-3′ Forward +639
a

The specific restriction site-tagged sequences are underlined, with their names in parentheses.

b

Position relative to transcription start site.