TABLE 1.
Name | Sequence of oligonucleotide | Orientation | Positionb |
---|---|---|---|
MMTR 1F (EcoRI) | 5′-GAATTCTA ATGGCTACGGGC-3′ | Forward | +58 |
MMTR 1R (XhoI) | 5′-CTCGAGAGCATCAAACACTGG-3′ | Reverse | +718 |
MMTR 2F (EcoRI) | 5′-GAATTCTA GGGCATGAGAGACGG-3′ | Forward | +719 |
MMTR 2R (XhoI) | 5′-CTCGAGTGG TTTCTTGGCTTTC-3′ | Reverse | +1462 |
MMTR 1F (NotI) | 5′-GCGGCCGCATGGCTACGGGC-3′ | Forward | +58 |
MMTR 2F (NotI) | 5′-GCGGCCGCGGGCATGAGAGACGG-3′ | Forward | +719 |
MAT1 primer1 (EcoRI) | 5′-CTGAATTCATGGACGATCAGGG-3′ | Forward | +102 |
MAT1 primer2 (XbaI) | 5′-TCTAGATTAACTGGGCTGCC-3′ | Reverse | +1029 |
MAT1 primer3 (XbaI) | 5′-TCTAGAATCCACATTGTTGG-3′ | Reverse | +444 |
MAT1 primer4 (EcoRI) | 5′-CTGAATTCACCAACAATGTGGA-3′ | Forward | +429 |
MAT1 primer5 (XhoI) | 5′-CTCGAGTAGAATCTGCTGCAG-3′ | Reverse | +627 |
MAT1 primer6 (EcoRI) | 5′-CTGAATTCAAGCAGGCTTTTTTA-3′ | Forward | +639 |
The specific restriction site-tagged sequences are underlined, with their names in parentheses.
Position relative to transcription start site.