Figure 3.

Northern blot analysis of the stc1 transcript. (A) Expression of the stc1 gene in different wild-type tissues: 20 μg of total RNA from each tissue were resolved in a 1.2% agarose gel and hybridized sequentially with an stc1 probe and with a genomic rDNA probe from tobacco. (B) Expression of stc1 in wild-type and mutant leaves: 1 μg of juvenile leaf mRNA from several different genotypes was resolved in a 1.2% agarose gel and hybridized with an stc1 probe and with a maize actin cDNA probe. The following genotypes were compared: wild type (Stc1-McC), the deletion mutation sh-bz-X2, and the Ac insertion mutation stc1-m6087 in homozygous and heterozygous condition.