TABLE 4.

Eme1 acts downstream of Swi5 in meiotic crossover formation

Straina	R1b	R2b	Totalc	% ± SEd	cMe	Fold reductionf
WT	190	209	1,112	35.9 ± 1.4	63.3
swi5Δ	43	42	700	12.1 ± 1.2g	13.9	4.6
eme1Δ	12	2	899	1.6 ± 0.4	1.6	39.6
swi5Δ eme1Δ	57	39	900	10.7 ± 1.0g	12.0	5.3

^aData were obtained for crosses between ade6 and arg1 single mutants of the indicated backgrounds: WT (sz1663 × sz1058), swi5Δ (sz1710 × sz1493), eme1Δ (sz1503 × sz1686), and swi5Δ eme1Δ (sz1718 × sz1720). Table 1 shows the genotypes.

^bR1 is the total number of prototrophs and R2 is the total number of double auxotrophs found in each background.

^cThree independent crosses of at least two different isolates were carried out for each strain.

^dPercentage of total recombinants and standard error for proportions.

^eCalculated from the formula of Haldane (1919), x = −1/2ln(1 − 2R), where x is the genetic distance in morgans and R is the recombinant fraction.

^fThe ratio of average centimorgans in the WT to average centimorgans in each mutant strain.

^gThe difference between these two values is not statistically significant. Analysis was done by χ², P = 0.36.