Skip to main content
. 2007 Mar 5;27(10):3625–3639. doi: 10.1128/MCB.02295-06

FIG. 1.

FIG. 1.

Activation-induced ROS generation depends on the proximal TCR signaling machinery. (A and B) Jurkat J16-145, P116 (ZAP70-negative Jurkat), P116cl.39 (ZAP70-retransfected control), J.CaM2 (LAT-negative Jurkat), J.CaM2/LAT (LAT-retransfected control), J14 (SLP76-deficient Jurkat), J14 76-11 (SLP76-retransfected control), J.γ1 (PLCγ1-deficient Jurkat), and J.γ1/PLCγ1 (PLCγ1-retransfected control) cells were stimulated via plate-bound anti-CD3 antibodies (A) or with PMA (B) for 30 min. Thereafter, cells were stained with DCFDA. Representative FACS profiles for activation-induced DCFDA oxidation are shown. ctr, retransfected control, def. cell lines, lines deficient in signaling molecules. (C) Schematic diagram of TCR signaling.