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. 2007 Apr 2;27(11):4093–4104. doi: 10.1128/MCB.01978-06

FIG. 6.

FIG. 6.

Ptf1a-containing complex binds area III and requires both the E and TC boxes. (A) Gel shift using the putative PTF1 binding site from area III or the PTF1 binding site from the elastase (Ela1) promoter as a control (closed arrowhead refers to specific complex). The addition of Ptf1a antibody (Ab) results in a complete supershift of the PTF1 complex bound to both the AIII probe and Ela1 PTF1 control probe (open arrowhead). The triangles depict increasing amounts of cold competitor: 10-, 50-, and 100-fold. The rectangle represents 100-fold competitor. Panc nuc, pancreatic nuclear extract; Min6 nuc, MIN-6 nuclear extract. (B) Gel shift competition experiment using indicated E box- or TC box-mutant cold competitors. The triangle depicts increasing amounts of cold competitor: 10-, 100-, and 1,000-fold. The rectangles represent 1,000-fold cold competitor. (C) Sequences of oligonucleotides used in the gel shift experiments. Dashes indicate gaps, periods indicate homology to AIII PTF1 probe, and capital letters indicate conserved nucleotides. Dup, duplication of spacer sequence.