Skip to main content
. 2007 Mar 12;27(11):4049–4057. doi: 10.1128/MCB.02023-06

FIG. 5.

FIG. 5.

Activation of ExPa gene expression by depletion of DNA methylation in HCT116 cells. (A) Bisulfite genomic sequencing of the TFF2 promoter shows reduced DNA methylation in methylation-deficient HCT116 cells carrying mutations in DNMT1 and DNMT3 (DKO cells) compared to wild-type HCT116 cells. CpGs are represented by vertical lines. Dotted lines show PCR products amplified after bisulfite treatment. Empty circles represent unmethylated cytosine residues, and filled circles represent methylated cytosine residues. (B) Real-time RT-PCR for ExPa genes in wild-type HCT116 and DKO cells. (C) Bisulfite genomic sequencing of the p48 promoter CpG island shows dense CpG methylation in HCT116 cells but not in DKO cells or human blood. CpGs are represented by vertical lines. The bracket (Bis) shows PCR products amplified after bisulfite treatment.