FIG. 6.

MBD2 binds to the TFF2 in a DNA methylation-dependent manner and reduces histone H4 acetylation levels. (A) Diagram indicating the location of CpG sites (vertical lines) and the TFF2 upstream fragments amplified by primer pairs A, B, and C. (B) ChIP shows reduced binding of MBD2 in regions A to C of the TFF2 promoter after either MBD2 depletion by RNA interference or depletion of DNA methylation in DNA methyltransferase mutant DKO cells. (C) Increased histone H4 acetylation after depletion of MBD2 or DNA methylation. ChIP analysis for panacetylated histone H4 at the TFF2 promoter was carried out with chromatin from wild-type and TSA-treated HCT116 cells, HCT116 cells expressing shRNA against MBD2, and DKO cells. Negative control immunoprecipitations in panels B and C (Co) were performed with an unrelated antibody (anti-α-amylase).