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p53 upregulated by Mdm2 siRNA fails to induce cell cycle arrest in U2OS cells. U2OS cells were transfected with control or Mdm2 siRNA or treated with doxorubicin (DOX) as indicated. At 24 and 48 h after transfection, cell lysates were immunoblotted (A) and corresponding dishes were pulsed with BrdU and subjected to staining and flow cytometry (B). The percentages of BrdU-positive cells are indicated at the top right corner of the panels.
