FIG. 2.

Distinct sets of HDACs are associated with LHβ and FSHβ genes in the immature gonadotropes. (A) ChIP analysis was carried out in duplicate to determine which HDACs are associated with the LHβ and FSHβ gene promoters in immature αT3-1 cells; an aliquot from the same cells before precipitation was designated as the input sample. PCR amplified a 567-bp or 300-bp region of the LHβ or FSHβ proximal promoters, respectively. (B) The ChIP analysis was repeated as relevant for HDACs 1 to 5 on LHβ (upper panels) or FSHβ (lower panels) gene promoters, following a 6-h GnRH exposure; the treatments were carried out in duplicate. Ab, antibody.