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P-body integrity depends on functional silencing pathways. S2 cells from which proteins involved in different steps of RNAi or the miRNA pathway were depleted were stained with affinity-purified anti-Tral or ant-Ge-1 antibodies. The fraction of cells exhibiting a staining identical to that shown in the representative panel was determined by scoring at least 100 cells in each of the three independent knockdowns performed per protein. Scale bar, 5 μm.
