FIG. 2.

Thermal repositioning of H3 truncated nucleosomes. (A) Cartoon illustration of the nucleosome core particle and the relative positions of residues R26, historically considered the last tail residue, and K37, which is the last residue. (B to E) Two picomoles of nucleosomes with either full-length or the indicated truncated H3 was incubated for 1 h at the temperatures indicated. Deletion of the entire H3 tail results in the nucleosomes' becoming diffusely distributed about the start position rather than moving to a new location.