FIG. 2.

DCLs posttranscriptionally regulate the steady-state levels of QDE-2, and dsRNA, but not siRNA, is responsible for the transcriptional activation of qde-2. (A) Western blot analysis showing that the level of QDE-2 could not be induced by dsRNA in the dcl double mutant. The liquid cultures were grown for 2 days (with/without QA) before harvesting. (B) Northern and Western blot analyses showing the induction of qde-2 expression by dsRNA in the wild-type (wt) and dcl double mutant strains. The number of hours indicates the time after the addition of QA. The asterisk indicates a nonspecific cross-reacting protein band recognized by our QDE-2 antibody.